ptakn-2 vector (BioDynamics Laboratory Inc)
90
Structured Review
BioDynamics Laboratory Inc
ptakn-2 vector
Ptakn 2 Vector, supplied by BioDynamics Laboratory Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptakn-2+vector/ptakn+2+vector/pm38483476-59-20-14
Average 90 stars, based on 1 article reviews
Ptakn 2 Vector, supplied by BioDynamics Laboratory Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptakn-2+vector/ptakn+2+vector/pm38483476-59-20-14
Average 90 stars, based on 1 article reviews
ptakn-2 vector - by Bioz Stars,
2026-09
90/100 stars
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Amplification:Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan. Article Snippet: The amplified products of blaOXA-1176 and blaOXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: Genetically-modified cells and method for producing same Article Snippet: DNAs artificially synthesized in Example 2 (SEQ ID NOs: 29 to 31) and SNC1-C (Takara Bio Inc., SEQ ID NO: 32) having sequences to be cleaved with MunI (MfeI) and ClaI added were integrated into a pTAKN-2 vector (BioDynamics Laboratory Inc.) or pBAsi vector (Takara Bio Inc.). Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan Article Snippet: The amplified products of bla OXA-1176 and bla OXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: DEVELOPMENT OF SNP MARKERS FOR MARKER-ASSISTED SELECTION IN PEAR Article Snippet: Single-nucleotide polymorphisms (SNPs) are desirable DNA markers for marker-assisted selection because of their high abundance, codominant inheritance, locus specificity and potential for automated high-throughput analysis.. The average number of SNPs in introns has been reported to be several times higher than that in exons in many organisms.. Potential intron polymorphism (PIP) markers, designed from expressed sequence tag (EST) sequences (exon regions), could amplify potential flanking intron regions without requiring genome sequence information. Plasmid Preparation:Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan. Article Snippet: The amplified products of blaOXA-1176 and blaOXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: Genetically-modified cells and method for producing same Article Snippet: DNAs artificially synthesized in Example 2 (SEQ ID NOs: 29 to 31) and SNC1-C (Takara Bio Inc., SEQ ID NO: 32) having sequences to be cleaved with MunI (MfeI) and ClaI added were integrated into a pTAKN-2 vector (BioDynamics Laboratory Inc.) or pBAsi vector (Takara Bio Inc.). Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan Article Snippet: The amplified products of bla OXA-1176 and bla OXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: DEVELOPMENT OF SNP MARKERS FOR MARKER-ASSISTED SELECTION IN PEAR Article Snippet: Single-nucleotide polymorphisms (SNPs) are desirable DNA markers for marker-assisted selection because of their high abundance, codominant inheritance, locus specificity and potential for automated high-throughput analysis.. The average number of SNPs in introns has been reported to be several times higher than that in exons in many organisms.. Potential intron polymorphism (PIP) markers, designed from expressed sequence tag (EST) sequences (exon regions), could amplify potential flanking intron regions without requiring genome sequence information. Transformation Assay:Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan. Article Snippet: The amplified products of blaOXA-1176 and blaOXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: Genetically-modified cells and method for producing same Article Snippet: DNAs artificially synthesized in Example 2 (SEQ ID NOs: 29 to 31) and SNC1-C (Takara Bio Inc., SEQ ID NO: 32) having sequences to be cleaved with MunI (MfeI) and ClaI added were integrated into a pTAKN-2 vector (BioDynamics Laboratory Inc.) or pBAsi vector (Takara Bio Inc.). Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan Article Snippet: The amplified products of bla OXA-1176 and bla OXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: DEVELOPMENT OF SNP MARKERS FOR MARKER-ASSISTED SELECTION IN PEAR Article Snippet: Single-nucleotide polymorphisms (SNPs) are desirable DNA markers for marker-assisted selection because of their high abundance, codominant inheritance, locus specificity and potential for automated high-throughput analysis.. The average number of SNPs in introns has been reported to be several times higher than that in exons in many organisms.. Potential intron polymorphism (PIP) markers, designed from expressed sequence tag (EST) sequences (exon regions), could amplify potential flanking intron regions without requiring genome sequence information. Synthesized:Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan. Article Snippet: The amplified products of blaOXA-1176 and blaOXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: Genetically-modified cells and method for producing same Article Snippet: DNAs artificially synthesized in Example 2 (SEQ ID NOs: 29 to 31) and SNC1-C (Takara Bio Inc., SEQ ID NO: 32) having sequences to be cleaved with MunI (MfeI) and ClaI added were integrated into a pTAKN-2 vector (BioDynamics Laboratory Inc.) or pBAsi vector (Takara Bio Inc.). Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan Article Snippet: The amplified products of bla OXA-1176 and bla OXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: DEVELOPMENT OF SNP MARKERS FOR MARKER-ASSISTED SELECTION IN PEAR Article Snippet: Single-nucleotide polymorphisms (SNPs) are desirable DNA markers for marker-assisted selection because of their high abundance, codominant inheritance, locus specificity and potential for automated high-throughput analysis.. The average number of SNPs in introns has been reported to be several times higher than that in exons in many organisms.. Potential intron polymorphism (PIP) markers, designed from expressed sequence tag (EST) sequences (exon regions), could amplify potential flanking intron regions without requiring genome sequence information. Purification:Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan. Article Snippet: The amplified products of blaOXA-1176 and blaOXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: Genetically-modified cells and method for producing same Article Snippet: DNAs artificially synthesized in Example 2 (SEQ ID NOs: 29 to 31) and SNC1-C (Takara Bio Inc., SEQ ID NO: 32) having sequences to be cleaved with MunI (MfeI) and ClaI added were integrated into a pTAKN-2 vector (BioDynamics Laboratory Inc.) or pBAsi vector (Takara Bio Inc.). Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan Article Snippet: The amplified products of bla OXA-1176 and bla OXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: DEVELOPMENT OF SNP MARKERS FOR MARKER-ASSISTED SELECTION IN PEAR Article Snippet: Single-nucleotide polymorphisms (SNPs) are desirable DNA markers for marker-assisted selection because of their high abundance, codominant inheritance, locus specificity and potential for automated high-throughput analysis.. The average number of SNPs in introns has been reported to be several times higher than that in exons in many organisms.. Potential intron polymorphism (PIP) markers, designed from expressed sequence tag (EST) sequences (exon regions), could amplify potential flanking intron regions without requiring genome sequence information. Polymerase Chain Reaction:Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan. Article Snippet: The amplified products of blaOXA-1176 and blaOXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: Genetically-modified cells and method for producing same Article Snippet: DNAs artificially synthesized in Example 2 (SEQ ID NOs: 29 to 31) and SNC1-C (Takara Bio Inc., SEQ ID NO: 32) having sequences to be cleaved with MunI (MfeI) and ClaI added were integrated into a pTAKN-2 vector (BioDynamics Laboratory Inc.) or pBAsi vector (Takara Bio Inc.). Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan Article Snippet: The amplified products of bla OXA-1176 and bla OXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: DEVELOPMENT OF SNP MARKERS FOR MARKER-ASSISTED SELECTION IN PEAR Article Snippet: Single-nucleotide polymorphisms (SNPs) are desirable DNA markers for marker-assisted selection because of their high abundance, codominant inheritance, locus specificity and potential for automated high-throughput analysis.. The average number of SNPs in introns has been reported to be several times higher than that in exons in many organisms.. Potential intron polymorphism (PIP) markers, designed from expressed sequence tag (EST) sequences (exon regions), could amplify potential flanking intron regions without requiring genome sequence information. Clone Assay:Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan. Article Snippet: The amplified products of blaOXA-1176 and blaOXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: Genetically-modified cells and method for producing same Article Snippet: DNAs artificially synthesized in Example 2 (SEQ ID NOs: 29 to 31) and SNC1-C (Takara Bio Inc., SEQ ID NO: 32) having sequences to be cleaved with MunI (MfeI) and ClaI added were integrated into a pTAKN-2 vector (BioDynamics Laboratory Inc.) or pBAsi vector (Takara Bio Inc.). Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan Article Snippet: The amplified products of bla OXA-1176 and bla OXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: DEVELOPMENT OF SNP MARKERS FOR MARKER-ASSISTED SELECTION IN PEAR Article Snippet: Single-nucleotide polymorphisms (SNPs) are desirable DNA markers for marker-assisted selection because of their high abundance, codominant inheritance, locus specificity and potential for automated high-throughput analysis.. The average number of SNPs in introns has been reported to be several times higher than that in exons in many organisms.. Potential intron polymorphism (PIP) markers, designed from expressed sequence tag (EST) sequences (exon regions), could amplify potential flanking intron regions without requiring genome sequence information. Isolation:Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan. Article Snippet: The amplified products of blaOXA-1176 and blaOXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: Genetically-modified cells and method for producing same Article Snippet: DNAs artificially synthesized in Example 2 (SEQ ID NOs: 29 to 31) and SNC1-C (Takara Bio Inc., SEQ ID NO: 32) having sequences to be cleaved with MunI (MfeI) and ClaI added were integrated into a pTAKN-2 vector (BioDynamics Laboratory Inc.) or pBAsi vector (Takara Bio Inc.). Article Title: Complete sequence of carbapenem-resistant Ralstonia mannitolilytica clinical isolate co-producing novel class D β-lactamase OXA-1176 and OXA-1177 in Japan Article Snippet: The amplified products of bla OXA-1176 and bla OXA-1177 genes were subcloned into the pTAKN-2 vector (BioDynamics Laboratory Inc., Tokyo, Japan) carrying kanamycin resistance gene and chemically transformed into ECOS X Competent Escherichia coli DH5α (Nippon Gene, Tokyo, Japan). Article Title: DEVELOPMENT OF SNP MARKERS FOR MARKER-ASSISTED SELECTION IN PEAR Article Snippet: Single-nucleotide polymorphisms (SNPs) are desirable DNA markers for marker-assisted selection because of their high abundance, codominant inheritance, locus specificity and potential for automated high-throughput analysis.. The average number of SNPs in introns has been reported to be several times higher than that in exons in many organisms.. Potential intron polymorphism (PIP) markers, designed from expressed sequence tag (EST) sequences (exon regions), could amplify potential flanking intron regions without requiring genome sequence information. |